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Watch this video to see why this paper is important: .https://www.youtube.com/watch?v=lIE_UElOk3c
I picked this paper: Host Cell Entry of Respiratory Syncytial Virus Involves Macropinocytosis Followed by Proteolytic Activation of the F Protein by Krzyzaniak et al, in PLoS Pathogens last week because of the depth and detail that they went to in order to determine one of the most basic and important processes in virus infection: cell entry.
How important is it to find out how a virus enters a cell?
Could purification/concentration of the virus particles change how the virus enters?
Did they use the correct cells (not primary respiratory cells) and virus (not a clinical isolate) for their experiments?
Would you target this pathway as an antiviral strategy?
What would you do next?
So people here is next week’s paper for discussion.
The wonderful images produced by the Hengge lab while dissecting the role of c-di-GMP in biofilm formation won. You will need to use the online material as well as the paper for this one.
We have discussed c-di-GMP and Biofilms sometime back and it is a subject that started my own journey into the fascinating world of Caulobacter crescentus as a young Post-Doc. Regine Hengge gave an excellent talk on this story at BACNET. The field of c-di-GMP itself played a central role at this meeting. What was clear to me is that the field has begun to move on from the biochemical characterisation of the components involved to returning to the underlying question of what c-di-GMP is regulating, why and how? Clearly exciting times are ahead for the field.
As per usual our discussion will focus on:
1) Did the paper read well?
2) Could you understand the regulatory network?
3) Would you have done anything differently?
4) where next?