Hi all

Zoonotica here posting this on Phil (@wragbags)’s behalf:

“I have had a few papers on my radar for my contribution the #microtwjc. In the end a paper that has changed my perspective on a number of things won. These include the impact MultiLocus Sequencing can have on diagnostics and the suggested costs of such tests. At our next meeting on July 30th we are going to discuss and dissect ‘Multilocus Sequence Typing as a Replacement for Serotyping in Salmonella enterica

Here is the Authors’ summary

Microbiologists have used serological and nutritional characteristics to subdivide pathogenic bacteria for nearly 100 years. These subdivisions in Salmonella enterica are called serovars, some of which are thought to be associated with particular diseases and epidemiology. We used MultiLocus Sequence-based Typing (MLST) to identify clusters of S. enterica isolates that are related by evolutionary descent. Some clusters correspond to serovars on a one to one basis. But many clusters include multiple serovars, which is of public health significance, and most serovars span multiple, unrelated clusters. Despite its broad usage, serological typing of S. enterica has resulted in confusing systematics, with a few exceptions. We recommend that serotyping for strain discrimination of S. enterica be replaced by a DNA-based method, such as MLST. Serotyping and other non-sequence based typing methods are routinely used for detecting outbreaks and to support public health responses. Moving away from these methods will require a major shift in thinking by public health microbiology laboratories as well as national and international agencies. However, a transition to the routine use of MLST, supplemented where appropriate by even more discriminatory sequence-based typing methods based on entire genomes, will provide a clearer picture of long-term transmission routes of Salmonella, facilitate data transfer and support global control measures.

Discussion Points

* Can this really replace traditional methods and when will that happen on the scale proposed?

* Where do you see this style of project going next?

* Was the presentation of such a complex subject done in an understandable manner?

* Experimentally would you have added anything to this story?”

We look forward to seeing/tweeting you all on Tues 31st July 🙂

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